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. 2024 Aug 20;31(11):1789–1797. doi: 10.1038/s41594-024-01373-9

Extended Data Fig. 4. Additional data on the interaction of MeCP2 RTT mutants with DNA.

Extended Data Fig. 4

a, Representative kymographs of unmethylated (top) and methylated (bottom) λ DNA tethers incubated with 2 nM Cy3-labeled MeCP2 or three RTT variants (T158M, P225R, R270X). b, Diffusion coefficients (D) for WT (n = 46 from 22 independent tethers), T158M (n = 11 from 6 independent tethers), P225R (n = 17 from 10 independent tethers), and R270X (n = 20 from 5 independent tethers) Cy3-MeCP2 trajectories on unmethylated DNA. Bars represent mean and s.e.m. Significance was calculated using a two-tailed unpaired t-test with Welch’s correction for each pair. c, Diffusion coefficients (D) for MeCP2P225R on unmethylated (n = 17 from 10 independent tethers) and methylated (n = 25 from 10 independent tethers) DNA. Bars represent mean and s.e.m. Significance was calculated using a two-tailed unpaired t-test with Welch’s correction.

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