Fig. 2. RTT mutations differentially alter MeCP2’s behavior on DNA.

a, Domain structure of MeCP2 and the corresponding PONDR disorder score. Three RTT mutants (T158M, P225R and R270X) are shown below. b, Loading efficiency for 2 nM Cy3-labeled MeCP2 (n = 22 independent tethers), MeCP2^T158M (n = 6 independent tethers), MeCP2^P225R (n = 10 independent tethers) and MeCP2^R270X (n = 5 independent tethers) binding to unmethylated DNA, and for 2 nM Cy3-labeled MeCP2 (n = 18 independent tethers), MeCP2^T158M (n = 9 independent tethers), MeCP2^P225R (n = 10 independent tethers) and MeCP2^R270X (n = 5 independent tethers) binding to CpG methylated DNA. Each dot represents data from one independent tether. The bars represent mean and s.d. c, Average MSD plot for Cy3-labeled MeCP2 (n = 78 from 22 independent tethers), MeCP2^T158M (n = 20 from 6 independent tethers), MeCP2^P225R (n = 40 from 10 independent tethers) and MeCP2^R270X (n = 44 from 5 independent tethers) trajectories on unmethylated DNA. The error bars represent s.d. d, Estimated number of molecules per trajectory for Cy3-labeled MeCP2 (n = 77 from 22 independent tethers), MeCP2^T158M (n = 20 from 6 independent tethers), MeCP2^P225R (n = 42 from 10 independent tethers) and MeCP2^R270X (n = 44 from 5 independent tethers) on unmethylated DNA. The bars represent mean and s.d. The significance for b and d was calculated using a one-way ANOVA with Tukey’s test for multiple comparisons.

Source data