Extended Data Fig. 1. MeCP2 constructs used in this work.

a, (Left) EMSA assay showing the products of 50 nM of 207-bp unmethylated DNA incubated with an increasing concentration of MeCP2 containing all three native cysteines or the single-cysteine version (C339S, C413S) of MeCP2. The latter construct was referred to as MeCP2 in this study. (Right) SDS–PAGE gel for Cy3-labeled MeCP2 (left lane: protein ladder; middle lane: Coomassie Blue staining; right lane: fluorescence scanning). b, Mass distribution for Cy3-labeled MeCP2 obtained by mass photometry with peak value indicated. c, SDS–PAGE gels for Cy3-MeCP2^T158M (left), Cy3-MeCP2^P225R (middle), and Cy3-MeCP2^R270X (right). d, SDS–PAGE gels for Cy3-MeCP2^K210X (left) and Cy3-MeCP2^R162X (right).

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