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. 2024 Nov 14;81(1):451. doi: 10.1007/s00018-024-05487-7

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — SPOP-induced RIPK3 K48 ubiquitination reduces RIPK3 half-life. A Amino acid sequence analysis of the RIPK3 showed putative degron motifs for the SPOP, A/V/P-X-S/T-S/T-S/T. B Screening of RIPK3 interaction with cullin 3 composing E3 ligases by IP in HEK293T cells. C Specific interaction between RIPK3 and SPOP was confirmed by IP in HEK293T cells. D IP confirmed the endogenous interaction of RIPK3 with SPOP in HT-29 cells. E RIPK3 protein levels were decreased by SPOP expression in a dose-dependent manner in HEK293T cells. F Top panels, RIPK3 half-life was shortened by the coexpression of SPOP in HeLa cells. Graphs, Normalized RIPK3 band intensities corresponding β-actin intensity. G Top panels, Knockout SPOP by Crispr-Cas9-SPOP extended RIPK3 half-life in HT-29 cells. Graphs, Normalized RIPK3 band intensities corresponding β-actin intensity. H RIPK3 ubiquitination in the endogenous levels was decreased in Crispr-Cas9-SPOP knockout HT-29 cells. I SPOP overexpression induced RIPK3 ubiquitination in HEK293T cells. J SPOP knockdown abolished RIPK3 ubiquitination in HEK293T cells. K In vitro RIPK3 ubiquitination assay. L Confirmation of the K48-ubiquitination of RIPK3 was mediated by SPOP in HEK293T cells. B-C and E-L β-actin was used as an internal control for an equal protein loading. F and G The error bars from three independent experiments indicate the SEM. * p < 0.05 (Student t-test).