KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Mouse anti-myc-tag (9B11) antibody | Cell Signaling Technology | Cat#2276S; RRID:AB_331783 |
| Mouse monoclonal anti-V5 tag antibody | Abcam | Cat#Ab27671; RRID:AB_471093 |
| Goat anti-mouse IgG Alexa Fluor 488 | Invitrogen | Cat#A11001; RRID:AB_2534069 |
| Bacterial and virus strains | ||
| DH5α competent cells | Thermo Fisher Scientific | Cat#18265017 |
| Chemicals, peptides, and recombinant proteins | ||
| 10x Phosphate Buffered Saline (PBS) | Fisher Scientific | Cat# BP39920 |
| Bovine Serum Albumin | Fisher Scientific | Cat#BP9706100 |
| Poly(ethylene glycol) 3350 | Sigma Aldrich | Cat#P4338 |
| Lithium acetate | Fisher Scientific | Cat#AC268640010 |
| Trizma base | Sigma Aldrich | Cat#T1503-1KG |
| Ethylenediaminetetraacetic Acid | Fisher Scientific | Cat#O2793-500 |
| Agarose | Fisher Scientific | Cat#BP160-500 |
| TAE Buffer | Fisher Scientific | Cat#BP13324 |
| Ovalbumin | Sigma Aldrich | Cat#A5503 |
| Biotinylated Soluble cytosolic proteins from CHO cells | Makowski et al.60 | N/A |
| Biotinylated Soluble membrane proteins from CHO cells | Makowski et al.60 | N/A |
| Insulin | Sigma Aldrich | Cat#I9278 |
| Sulfo-NHS-LC-biotin | Thermo Fisher Scientific | Cat#21335 |
| Streptavidin Alexa Fluor 647 | Invitrogen | Cat# S32357 |
| NeutrAvidin PE | Invitrogen | Cat#A2660 |
| Dextrose (D-Glucose) | Fisher Scientific | Cat#D16-10 |
| Yeast Nitrogen Base without Amino Acids | Fisher Scientific | Cat#DF0919-15-3 |
| Casamino Acids | Fisher Scientific | Cat#BP1424-500 |
| Sodium Citrate Dihydrate | Fisher Scientific | Cat#S279-500 |
| Citric Acid | Fisher Scientific | Cat#A940-500 |
| D(+)-Galactose | Fisher Scientific | Cat#AC150610051 |
| Sodium Phosphate Monobasic Monohydrate | Fisher Scientific | Cat#S369-500 |
| Sodium Phosphate Dibasic Dihydrate | Fisher Scientific | Cat#S472-500 |
| Lithium acetate | Fisher Scientific | Cat# AC268640010 |
| Critical commercial assays | ||
| Zymoprep Yeast Plasmid Miniprep II | Zymo Research | Cat#D2004 |
| QIAquick Gel Extraction Kit | QIAGEN | Cat# 28704 |
| Non-fat dry milk | Kroger | Cat#0001111008733 |
| Deposited data | ||
| Human Antibodies | This paper | Datasets S1, S2, S3, S4, S5, S6, S7, S8, and S9 |
| Original Code | This paper | https://doi.org/10.5281/zenodo.13387057 |
| Experimental models: cell lines | ||
| Yeast: EBY100 | Julian et al.61 | N/A |
| Recombinant DNA | ||
| pCTcon2 | Chao et al.62 | Addgene Cat#41843 |
| Software and algorithms | ||
| Python version 3.9 | Python Software Foundation | https://www.python.org |
| RStudio Desktop version 2024.4.1.748 | Posit team | https://posit.co/download/rstudio-desktop |
| MATLAB R2019a | MathWorks | https://www.mathworks.com/products/matlab.html |
| Others | ||
| Beckman Coulter MoFlo Astrios | Beckman Coulter | Cat#B52102 |
| Protein G Dynabeads | Invitrogen | Cat# S311-500 |