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. 2024 Nov 14;14:28032. doi: 10.1038/s41598-024-78092-0

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

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Fig. 1 — UTI alleviated renal injury and interstitial fibrosis induced by UUO. (A) The renal injury and fibrosis were evaluated by H&E and Masson staining, respectively (Original magnification: × 400, scale: 20 μm, the black arrows indicate inflammatory cells). (B) The quantitative analysis of the area affected by renal interstitial fibrosis (**P < 0.01, ***P < 0.001, ****P < 0.0001). (C) The levels of Collagen I and α-SMA were detected by qPCR (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001). (D and E) The levels of Collagen I and α-SMA were detected by Western blot. Tubulin was used as the loading control (*P < 0.05, **P < 0.01, ***P < 0.001). (F and G) Immunohistochemical staining was performed to assess the expression of Collagen I and α-SMA at a cellular level, the positive areas were measured using Image J (Original magnification: × 400, scale: 20 μm, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001). Results were presented as mean ± SD of three individual experiments. The full-length blots/gels are presented in Supplementary Fig. 1.