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. 2024 Nov 14;14:28032. doi: 10.1038/s41598-024-78092-0

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

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Fig. 3 — UTI blocked the glycolysis process induced by UUO. (A) The expression of PKM2 and LDHA was detected by qPCR (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001). (B) The levels of PKM2 and LDHA were detected by Western blot. (C) The grey value results of PKM2 and LDHA. Tubulin was used as the loading control (*P < 0.05, **P < 0.01). (D) Mitochondrial morphology in three groups of mice, red arrows indicate mitochondria (Original magnification: × 20000, scale: 0.5 μm) (E and F) The expression of PKM2 was evaluated by immunohistochemical staining, the positive areas were quantified (Original magnification: × 400, scale: 20 μm, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001). (G) The expression of AMPK and HIF-1α was detected by qPCR (*P < 0.05, **P < 0.01, ***P < 0.001). (H) The levels of AMPK, p-AMPK and HIF-1α were detected by Western blot. (I) The analysis of grey value of p-AMPK and HIF-1α. AMPK and Tubulin were used as the loading controls (*P < 0.05, **P < 0.01). Results were presented as mean ± SD of three individual experiments. The full-length blots/gels are presented in Supplementary Fig. 1 and Fig. 3.