Figure 1.

VSMCs autophagy was induced by rapamycin. Expression of (A) P62 mRNA and (B) LC3 mRNA in VSMCs following treatment with rapamycin at different concentrations. (C-E) Expression of P62 and LC3 protein in VSMCs following treatment with rapamycin at different concentrations. (F) Expression of SNHG1 following treatment with rapamycin at different concentrations; (G) Morphological changes of VCMC following rapamycin treatment at the concentrations of 100 nM, scale bar, 50 µm. Data were shown as mean ± standard deviation of a representative experiment performed in three independent replicates. Compared with DMSO treatment *P<0.05, **P<0.01. VSMCs, vascular smooth muscle cells; SNHG1, small ribosome housekeeping gene RNA1; DMSO, dimethyl sulfoxide.