Figure 2.

SNHG1 silencing and overexpression vector transduction of VSMCs. (A) Fluorescence map of lentivirus transduced SNHG1 silencing sequence into cells. (B) RT-qPCR detection of SNHG1 expression in silenced cells. (C) Fluorescence pattern of cells transduced with lentivirus following overexpression of SNHG1. (D) RT-qPCR detected the expression of SNHG1 in the cells following overexpression. Scale bar: 200 µm. Data were shown as mean ± standard deviation of a representative experiment performed in three independent replicates. **P<0.01. SNHG1, small ribosome housekeeping gene RNA1; VSMCs, vascular smooth muscle cells; RT-qPCR, reverse transcription-quantitative PCR; sh, short hairpin; NC, negative control; OE, overexpression.