Table 3.

Differential methylation patterns in specific CpG sites within BDNF gene in relation to perceived stress and inflammatory cytokines.

GzLM models - Perceived Stress
	β	SE	95% CI		Wald ꭓ2	P-value	P-value adja
			Min	Max
DNA methylation (BDNF - CpGs %)
CpG5	0.143	0.063	0.019	0.267	5.092	0.024	0.033
CpG6	0.095	0.040	0.016	0.174	5.524	0.019	0.028
CpG7	−0.260	0.076	−0.409	−0.110	11.642	<0.001	0.017
CpG8	−0.196	0.042	−0.279	−0.113	21.435	<0.001	0.011
CpG9	−0.364	0.080	−0.521	−0.207	20.626	<0.001	0.009
CpG10	−0.962	0.198	−1.350	−0.573	23.555	<0.001	0.007
CpG11	0.398	0.115	0.173	0.624	11.965	<0.001	0.006
CpG12	0.767	0.169	0.437	1.098	20.692	<0.001	0.005
CpG13	0.410	0.094	0.226	0.594	19.117	<0.001	0.004
Inflamatory markers
IL-6 (pg/mL)	0.036	0.014	0.008	0.063	6.487	0.011	0.033
IL-8 (pg/mL)	0.049	0.013	0.024	0.074	15.189	<0.001	<0.0001
IL-10 (pg/mL)	−0.020	0.029	−0.077	0.038	0.442	0.506	0.759
IL-12p70 (pg/mL))	0.015	0.018	−0.021	0.050	0.651	0.420	0.944

Note: Generalized linear models obtained with Gamma distribution. P-values were presented based on Wald's chi-square test (Wald ꭓ2) and were adjusted by FDR (False Discovery Rate). P < 0.05 (IC = 95%); BMI: body mass index; HADS-A and HADS-D: Hospital Anxiety and Depression Scale; CpG: DNA methylation sites of BDNF; IL: interleukin.

^aFDR: False Dicovery Rate.