Key resources table
| REAGENT OR RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Chemicals | ||
| 3,3',5-Triiodo-L-thyronine | Cayman | CAS#345957-19-9 |
| Thyroid Hormone Receptor Antagonist (1-850) | Cayman | CAS#251310-57-3 |
| UNC1999 | Cayman | CAS#1431612-23-5 |
| GSK126 | Cayman | CAS#1346574-57-9 |
| EPZ-6438 | Cayman | CAS#1403254-99-8 |
| D-Luciferin | PerkinElmer Informatics Inc | Cat# 122799 |
| 2'-Deoxy-5-ethynyluridine (EdU) | Carbosynth | Cat# NE08701 |
| Tetramethylrhodamine (TAMRA) | Thermo Fisher | Cat# T10182 |
| Neurobasal Medium (NB) | Thermo Fisher | Cat# 21103049 |
| B27 supplement | Thermo Fisher | Cat# 17504044 |
| Antibodies | ||
| Rabbit polyclonal to Thyroid Hormone Receptor alpha | Abcam | Cat# ab53729; RRID: AB_882974 |
| Rabbit monoclonal anti-Ki67 | Abcam | Cat#ab15580; RRID: AB_443209 |
| Mouse monoclonal anti-NeuN | Abcam | Cat#ab104224; RRID: AB_10711040 |
| Rabbit monoclonal anti-Microtubule Associated Protein 2 (MAP2) | Millipore | Cat# AB5622; RRID: AB_91939 |
| Mouse monoclonal anti-beta III Tubulin | Abcam | Cat#ab78078; RRID: AB_2256751 |
| Rabbit monoclonal anti-Cleaved Caspase3 | Cell Signaling Technology | Cat#9664; RRID: AB_2070042 |
| Mouse monoclonal anti-Neurod1 | Abcam | Cat# ab60704; RRID: AB_943491 |
| Chicken Polyclonal anti-GFP | Thermo Fisher Scientific | Cat#A10262; RRID: AB_2534023 |
| Rabbit monoclonal anti-Histone H3, Trimethyl (Lys27) | Cell Signaling Technology | Cat#9733; RRID: AB_2616029 |
| Rabbit monoclonal anti-EZH2 | Cell Signaling Technology | Cat#5246; RRID: AB_10694683 |
| Rabbit monoclonal anti-GAPDH | Cell Signaling Technology | Cat#2118; RRID: AB_561053 |
| Goat anti-Rabbit IgG Alexa Fluor 488 | Thermo Fisher Scientific | Cat# A31566, RRID: AB_10374301 |
| Goat anti-Rabbit IgG Alexa Fluor 594 | Thermo Fisher Scientific | Cat# A11012; RRID: AB_2534079 |
| Goat anti-Mouse IgG Alexa Fluor 488 | Thermo Fisher Scientific | Cat# A-31560, RRID: AB_2536174 |
| Goat anti-Mouse IgG Alexa Fluor 594 | Thermo Fisher Scientific | Cat# A-11005, RRID: AB_2534073 |
| Goat Anti-Mouse HRP Conjugate | Bio-Rad | Cat# 170-5047, RRID: AB_11125753 |
| Goat Anti-Rabbit HRP Conjugate | Bio-Rad | Cat# 170-5046, RRID: AB_11125757 |
| Biological Samples | ||
| human MB PDX lines | Northwestern University | ICb-5610 |
| human MB PDX lines | Columbia university | Med1712 |
| human MB PDX lines | Columbia university | Rcmb20 |
| human MB PDX lines | Columbia university | Rcmb28 |
| human MB PDX lines | St Jude children's hospital | SJNBT12 |
| EZH2 Human Recombinant Protein | Origene Technologies Inc | Cat# TP302054 |
| Recombinant Human THRA Protein | Creative Biogene | Cat# THRA-437H |
| pU6-sgRNA#1TRα1-sgRNA#2TRα1 | VectorBuilder | Cat# VB211018-1217ptt |
| pU6-shRNA-TRα1 | VectorBuilder | Cat# VB211018-1218mrf |
| Experimental Models: Organisms/Strains | ||
| Mouse: Ptch1loxp/loxp | Jackson Laboratory | https://www.jax.org/strain/030494 |
| Mouse: Math1-Cre | Jackson Laboratory | https://www.jax.org/strain/011104 |
| Mouse: CB17/SCID | Jackson Laboratory | https://www.jax.org/strain/001303 |
| Mouse: C57BL/6J | Fox Chase Cancer Center | N/A |
| Critical commercial assays | ||
| BCA Protein Assay Kit | Thermo Fisher Scientific | Cat# 23225 |
| Pierce™ Magnetic ChIP Kit | Thermo Fisher Scientific | Cat# 26157 |
| Triiodothyronine (T3) ELISA | Thermo Fisher Scientific | Cat# EIAT3C |
| Others | ||
| Benchtop pH meter | Fisher Scientific | AB150 |
| Centrifuge | Thermo Scientific | ST40R |
| Inverted microscope | Zeiss | Axiovert 40 CFL |
| Small Animal Stereotaxic Frame | David Kopf Instruments | Model 900LS |
| Micromanipulator | David Kopf Instruments | Model 1760 |
| Replacement blade | Excel | Cat# 84-327-1 |
| Unbeveled Hamilton syringe | Hamilton | Cat# 76350-01 |
| Beveled 18-gauge needle | BD Bioscience | Cat# 5302622 |
| Nylon skin suture (with needle) | Fisher Scientific | Cat# NC1646047 |
| Deltaphase Isothermal Pad | Braintree Scientific | Cat# 39DP |
| Nutrition gel | ClearH2O | Cat# 72-06-5022 |