Figure 5. A role for the chromosome 10 inversion in sex determination.

(A) Schematic of the two inversion states (normal and inverted) and their assignment as X or Y chromosomes. (B) 24 offspring (12 male and 12 female) from two separate male/female pairs were genotyped for the inversion. For 47 of 48 individuals, the XY animals were male and the XX animals were female. One male was XX. (C) F_ST analysis comparing 24 males and 24 females identified chromosome 10 as the primary genetic region that segregated with sex. (D) Comparison of heterozygosity levels of 24 male and 24 female animals also identified chromosome 10 as an outlier.