Figure 5. TnC promotes MuSC migration in vitro.

(A) Representative images of time-lapse microscopy to track cultured MuSC from adult WT and TnC-KO mice. Scale bar= 50 µm.

(B) Quantification of total distance covered (n= 3, N_cell≥75).

(C) Quantification of average velocity of cells (n= 3, N_cell≥75).

(D) Quantification of migrated TnC-KO cells through transwell matrix normalized on WT (n=6).

(E) Quantification of the percentage of proliferating cells through EdU staining (timepoint= 48 hours) (n=3).

(F) Representative immunofluorescence (IF) images of migrated WT MuSC from adult (3–5 months old) mice with or without recombinant TnC treatment (EdU gray, DAPI blue) (scale bar= 50 µm). Quantification of normalized migrated TnC-KO cells through transwell matrix after TnC treatment (timepoint= 48 hours) (n=6) and quantification of the percentage of proliferating cells through EdU staining (timepoint= 48 hours) (n=3).

Data are represented as mean ± SEM; *p<0.05 and **p<0.01, t test.