Table 2. Twenty-Seven Most Highly Expressed Compounds Identified in Five Annual Ryegrass Cultivarsa.
| compound | RT | genotype (peak area × 104) |
RI | molecular formula | MW | ||||
|---|---|---|---|---|---|---|---|---|---|
| safeguard-I | safeguard-II | Tetila | Wimmera | wild type | |||||
| neophytadiene | 29.446 | 13,397 | 13,534 | 6487 | 5768 | 8374 | 1841 | C20H38 | 278.30 |
| palmitic acid | 31.981 | 10,275 | 1645 | n.d. | n.d. | 753 | 1967 | C16H32O2 | 256.24 |
| hexanedioic acid, dioctyl ester | 39.871 | 6427 | n.d. | 5459 | 5436 | 3350 | 2401 | C22H42O4 | 370.31 |
| tricosyl pentafluoropropionate | 40.432 | 10,039 | 7873 | 7801 | 5930 | 1680 | 2483 | C26H47F5O2 | 486.35 |
| tricosyl trifluoroacetate | 40.461 | 3545 | n.d. | 2614 | 2480 | n.d. | 2487 | C25H47F3O2 | 436.35 |
| 2-chloropropionic acid, octadecyl ester | 40.467 | n.d. | 4229 | n.d. | n.d. | n.d. | 2488 | C21H41ClO2 | 360.28 |
| carbonic acid, eicosyl vinyl ester | 40.57 | 4616 | 5658 | 9546 | 9264 | 9266 | 2503 | C23H44O3 | 368.32 |
| heptacos-1-ene | 41.771 | 11,997 | 1278 | 10,211 | 8574 | 4426 | 2682 | C27H54 | 378.42 |
| n-tetracosanol-1 | 41.834 | 7395 | n.d. | 6186 | 6073 | 10,645 | 2691 | C24H50O | 354.39 |
| tetracosanoic acid, methyl ester | 42.143 | 3051 | 796 | 318 | 826 | 295.84 | 2734 | C25H50O2 | 382.38 |
| tetracosyl acetate | 42.752 | n.d. | n.d. | n.d. | n.d. | 712.89 | 2814 | C26H52O2 | 396.40 |
| squalene | 42.973 | 2880 | 4395 | 3817 | n.d. | 6880 | 2840 | C30H50 | 410.39 |
| hexacosanal | 43.001 | n.d. | n.d. | n.d. | 4349 | n.d. | 2844 | C26H52O | 380.40 |
| heptacosyl heptafluorobutyrate | 43.619 | 4614 | 3424 | 13,968 | 11,790 | 8235 | 2915 | C31H55F7O2 | 592.40 |
| octacosanal | 45.41 | 4730 | n.d. | n.d. | n.d. | 915 | 3084 | C28H56O | 408.43 |
| myristic acid, 9-hexadecenyl ester, (Z)- | 45 | n.d. | 5967 | n.d. | n.d. | 2085 | 3085 | C30H58O2 | 450.44 |
| 2,3-bis(acetyloxy)propyl (9E,12E,15E)-9,12,15-octadecatrienoate | 45 | 7109 | n.d. | n.d. | n.d. | n.d. | 3097 | C25H40O6 | 436.28 |
| 9,12,15-octadecatrienoic acid, 2,3-dihydroxypropyl ester, (Z,Z,Z)- | 45.571 | n.d. | 7063 | n.d. | n.d. | n.d. | 3099 | C21H36O4 | 352.26 |
| fumaric acid, 8-chlorooctyl tridecyl ester | 45.719 | 1517 | n.d. | n.d. | n.d. | n.d. | 3210 | C25H45ClO4 | 444.30 |
| brassicasterol acetate | 45.902 | 1649 | 1044 | 588 | n.d. | n.d. | 3223 | C30H48O2 | 440.36 |
| octacosanoic acid, methyl ester | 46.085 | n.d. | n.d. | 1362 | 3091 | 680 | 3236 | C29H58O2 | 438.44 |
| Stigmasterol | 46.32 | 2592 | 1435 | n.d. | 1384 | 2013 | 3254 | C29H48O | 412.37 |
| cholest-4-en-3-one, 7-hydroxy-, (7β)- | 48.357 | 7748 | 6324 | 5302 | 2030 | 2650 | 3485 | C27H44O2 | 400.33 |
| stigmastane-3,6-dione, (5α)- | 48.863 | n.d. | n.d. | n.d. | n.d. | 3451 | 3613 | C29H48O2 | 428.36 |
| ergosterol peroxide | 48.912 | 12,939 | n.d. | n.d. | n.d. | n.d. | 3616 | C28H44O3 | 428.33 |
| ergosterol peroxide Ac (acetyl) derivative | 48.941 | n.d. | 13,654 | n.d. | 2085 | n.d. | 3617 | C30H46O4 | 470.34 |
| methyl nomilinate (isomer 2) | 49.198 | 1450 | 2493 | n.d. | n.d. | n.d. | 3630 | C29H38O10 | 546.25 |
a
RI-retention index; RT-retention time; n.d.-not detected; MW-molecular weight. Values represent the mean of three replicates.