Table 2. Comparison of Enzymes and Reaction Conditions for Theanderose Synthesis.
|
substrate (M) |
|||||||||
|---|---|---|---|---|---|---|---|---|---|
| enzyme | origin | reaction temperature (°C) | reaction pH | reaction time (h) | sucrose | isomaltose | residual sucrose (M) | theanderose productivity (g/L·h) | references |
| amylosucrase | Deinococcus deserti | 40 | 6.0 | 48 | 2.00 | 0 | 3.6 | this study | |
| levansucrase | Bacillus subtilis CECT39 | 37 | 6.0 | 3 | 0.61 | 0.59 | 0.26 | 42.1 | (9) |
| α-glucosidase | Metschnikowia reukaufii | 72 | 1.46 | 0.078 | (54) | ||||
| α-glucosidase | Bacillus subtilis SAM1606 | 60 | 6.0 | 48 | 1.75 | 1.3 | (34) | ||