Extended Data Figure 1. β-OHB is preferentially used over glucose for fatty acid synthesis, Related to Figure 2.

A-D, 16:0 MID (A, C) and citrate (solid bars) and cytosolic acetyl-CoA (dashed bars) MID (B, D) for AL1376 (A, B) and MIA PaCa-2 (C, D) cells labeled with [U-¹³C]-glucose for 48 h in lipid-replete versus lipid-depleted culture media without unlabeled β-OHB. E-H, 16:0 MID (E, G) and citrate (solid bars) and cytosolic acetyl-CoA (dashed bars) MID (F, H) for AL1376 (E, F) and MIA PaCa-2 (G, H) cells labeled with [U-¹³C]-glucose for 48 h in lipid-replete versus lipid-depleted culture media with 5 mM unlabeled β-OHB. Data are presented as mean ± s.e.m; n = 3 biologically independent replicates.