Extended Data Figure 2. β-OHB is preferentially used over glucose for PUFA elongation, Related to Figure 2.

A-C, [M+2] fractional labeling of 20:3(n-6), 20:4(n-6), 22:4(n-6), and 22:6(n-3) in B16 (A), AL1376 (B), and MIA PaCa-2 (C) cells labeled with [U-¹³C]-β-OHB, [U-¹³C]-glucose without unlabeled β-OHB, or [U-¹³C]-glucose with 5 mM unlabeled β-OHB for 48 h in lipid-replete media. Data are presented as mean ± s.e.m; n = 3 biologically independent replicates. Comparisons were made using a two-tailed Student’s t test (A-C). ***P<0.001.