Extended Data Figure 3. β-OHB can contribute to fatty acid synthesis through a citrate-independent route that requires AACS, Related to Figure 3.

A-E, Proliferation rates of the indicated B16 sgNTC (A), sgBdh1 (B), sgOxct1 (C), sgAacs (D), and sgOxct1/Aacs (E) cells grown in lipid-replete versus lipid-depleted culture media, with or without 5 mM β-OHB. Data are presented as mean ± s.e.m; n = 3 biologically independent replicates. Comparisons were made using a two-way ANOVA. ***P<0.001.