Extended Data Figure 4. β-OHB contributes to PUFA elongation through both OXCT1 and AACS, Related to Figure 3.

A, [M+2] fractional labeling of 20:3(n-6), 20:4(n-6), 22:4(n-6), and 22:6(n-3) in B16 sgNTC, sgBdh1 #1, sgOxct1 #1, and sgAacs #2 cells labeled with [U-¹³C]-β-OHB for 48 h in lipid-replete media. B, [M+2] fractional labeling of 20:3(n-6), 20:4(n-6), 22:4(n-6), and 22:6(n-3) in B16 sgNTC and sgOxct1/Aacs #1 cells labeled with [U-¹³C]-β-OHB for 48 h in lipid-replete media. Data are presented as mean ± s.e.m; n = 3 biologically independent replicates. Comparisons were made using a two-way ANOVA. ***P<0.001.