Table 1. ITC thermodynamic parameters.
(n is stoichiometry, Kd is dissociation constant). Values are shown as average of triplicate measurements and the errors are obtained as the standard deviation from the mean value (See Materials and Methods). NB: no binding.
| Experiment | n | Kb (M−1) | Kd (μM) | ΔH kcal/mol | −TΔS kcal/mol | ΔG kcal/mol |
|---|---|---|---|---|---|---|
| 575–625 : RACK1 | 1 | (3.7 ± 0.4)×105 | 2.7 ± 0.3 | −5.7 ± 0.1 | −1.9 ± 0.2 | −7.6 ± 0.1 |
| 587–637 : RACK1 | 1 | (4.4 ± 0.3)×105 | 2.3 ± 0.2 | −7.8 ± 0.1 | −0.2 ± 0.1 | −7.7 ± 0.1 |
| 600–625 : RACK1 | 1 | (3.9 ± 0.6)×105 | 2.5 ±0.4 | −5.2 ±0.2 | −2.4 ± 0.1 | −7.6 ± 0.1 |
| 600–650 : RACK1 | 0.9 | (4.7±0.5)×105 | 2.1 ± 0.3 | −7.2 ±0.2 | −0.5 ± 0.2 | −7.7 ± 0.1 |
| 550–600 : RACK1 | NB | NB | NB | NB | NB | NB |
| 615–625 :RACK1 | 0.9 | 3.4 ± 0.2 | 3.0 ± 0.1 | −8.1 ± 0.1 | −0.6 ± 0.1 | −7.5 ± 0.1 |
| 612–625 :RACK1 | 0.8 | 4.9 ± 0.1 | 2.0 ± 0.1 | −8.3 ± 0.1 | −0.6 ± 0.1 | −7.7 ± 0.1 |
| 612–637 :RACK1 | 0.9 | 4.8 ± 0.2 | 2.1 ± 0.1 | −8.6 ± 0.1 | −0.8 ± 0.1 | −7.8 ± 0.1 |