|
Bacterial and virus strains
|
| AAV9-CAG-FLEX-tdTomato |
Penn Vector Core |
AV-9-ALL864 |
| AAV8-CAG-GFP |
Addgene |
Addgene viral prep # 37825-AAV8, RRID: Addgene_37825 |
| AAV5-Syn-ChrimsonR-tdTomato |
Addgene |
Addgene viral prep # 59171-AAV5, RRID: Addgene_59171 |
| AAV5-hSyn-hChR2(H134R)-EYFP |
Addgene |
Addgene viral prep # 26973-AAV5, RRID: Addgene_26973 |
| AAV1-pENN.AAV.hSyn.Cre.WPRE.hGH |
Addgene |
Addgene viral prep # 105553-AAV1, RRID: Addgene_105553 |
| AAV1-EF1a-Flpo |
Addgene |
Addgene viral prep # 55637-AAV1, RRID: Addgene_55637 |
| AAV8-hSyn Con/Fon EYFP (AAV8-hSyn-cDIO-fDIO-EYFP) |
Addgene |
Addgene viral prep # 55650-AAV8, RRID: Addgene_55650 |
| AAV1-syn-FLEX-jGCaMP8m-WPRE |
Addgene |
Addgene viral prep # 162378-AAV1, RRID: Addgene_162378 |
| AAV5-hSyn1-SIO-eOPN3-mScarlet-WPRE |
Addgene |
Addgene viral prep # 125713-AAV5, RRID: Addgene_125713 |
| AAV8-hSyn-DIO-mCherry |
Addgene |
Addgene viral prep # 50459-AAV8, RRID: Addgene_50459 |
| AAVrg-pENN.AAV.hSyn.Cre.WPRE.hGH |
Addgene |
Addgene viral prep # 105553-AAVrg, RRID: Addgene_105553 |
| AAV-DJ-FLEX-taCasp3-TEVp (AAV-CASPASE3) |
Yang et al., 201365
|
N/A |
|
Chemicals, peptides, and recombinant proteins
|
| Picrotoxin |
Tocris |
Cat# 1128 |
| NBQX |
Tocris |
Cat# 0373 |
|
Experimental models: Organisms/strains
|
| Mouse: C57BL/6J (WT) |
The Jackson Laboratory |
JAX #:000664, RRID:IMSR_JAX:000664 |
| Mouse: B6J.129S6(FVB)-Slc32a1tm2(cre)Lowl/MwarJ (VGAT-Cre) |
The Jackson Laboratory |
JAX #:028862, RRID:IMSR_JAX: 028862 |
|
Recombinant DNA
|
| pAAV-CAG-RiboL1-jGCaMP8m |
Addgene |
Addgene plasmid # 192613, RRID: Addgene_192613 |
|
Software and algorithms
|
| MATLAB |
Mathworks |
https://www.mathworks.com; RRID:SCR_001622 |
| ImageJ/FIJI |
Schindelin et al., 201266
|
http://fiji.sc/; RRID:SCR_002285 |
| TrakEM2 |
Cardona et al., 201267
|
http://www.ini.uzh.ch/~acardona/trakem2.html/; RRID:SCR_008954 |
| napari |
Napari Contributors68
|
https://napari.org; RRID:SCR_022765 |
| BrainGlobe Atlas API |
Claudi et al., 202069
|
https://brainglobe.info; RRID:SCR_023848 |
| Allen Mouse Brain Common Coordinate Framework |
Allen Institute for Brain Science |
https://atlas.brain-map.org/; RRID:SCR_020999 |
| PyCharm |
Jetbrains |
https://www.jetbrains.com/pycharm/; RRID:SCR_018221 |
| Visual Studio Code |
Microsoft |
https://code.visualstudio.com/
|
| ZEISS ZEN Microscopy Software |
Zeiss |
https://www.zeiss.com; RRID:SCR_013672 |
| LabView |
NI |
http://www.ni.com/labview/; RRID:SCR_014325 |
| Excel |
Microsoft |
https://www.microsoft.com/; RRID:SCR_016137 |
| Prism 10 |
GraphPad Software |
http://www.graphpad.com; RRID:SCR_002798 |
| Illustrator |
Adobe |
https://www.adobe.com/products/illustrator.html; RRID:SCR_010279 |
| Custom scripts for analysis |
This study |
|
