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[Preprint]. 2024 Nov 1:2024.11.01.621496. [Version 1] doi: 10.1101/2024.11.01.621496

Figure 2. Mitochondria defects in iN cells derived from OCRL-deficient iPSCs.

Figure 2.

(a) Quantitative real-time PCR (RT-PCR) validation of mitochondrial DNA genes COX2 and DLOOP in iN cells. RT-PCR was repeated three times with different batches. Gene expression values are normalized to ACTIN. (b and c) iN cells derived from Lowe syndrome iPSCs and OCRL knockout iPSCs stained with 8-oxo-dg (red) antibodies and expressed Ngn2-EGAP. DNA stained with DAPI (blue). Scale bars are as indicated. (d) Quantification of the percentage of iPSCs-derived iN cells positive for 8-oxo-dg signal. > 100 cells analyzed for each independent experiment. (e) Oxygen consumption rate of Lowe syndrome iPSCs-derived iN cells and OCRL knockout iPSCs-derived iN cells measured by Seahorse Analyzer. The bars in each graph represent mean ± SD. Statistical significance was determined using Student’s t-test, with exact p-values reported.