Fig. 5. Temperature modulation across the tuft dendrite causes somatic rate regulation.

A) Schematic of the dynamic clamp circuit that generates in-vivo-like conductance state through a feedback loop.

B) Cooling effect on the intrinsic burstiness when a subset of bAPs is paired with apical excitatory conductance without (top block) and with cooling (bottom block).

C) Histogram of the probability of the normalized I.S.I of the spikes in the presence of dendritic input without and with cooling.

D) The ratio of the number of the burst to the total number of the spikes defined as burst probability is presented as function of the temperature when a dendritic input is coincident with a bAP (Wilcoxon signed rank test, n=6 neurons, p=0.0313) and the mean ISI (Wilcoxon signed rank test, n=7 neurons, p=0.6875). Note: a burst is defined as two consecutive spikes with the ISI smaller than 50 ms.

E) Spiking activities of a three-compartment biophysical model under apical dendritic focal cooling (top) and physiological temperature (bottom), gray traces indicate corresponding dendritic voltage.

F) Burst probability is reduced under apical focal cooling compared to physiological temperature (n = 14, the variance is due to imposed modulation in the dendritic geometry across models, see Methods for details, Wilcoxon signed rank test, p < 0.005).