Table 3.

Repeatability test of the qPCR-HRM assay.

Viruses	Concentration of plasmid standards(copies/μL−1)	Intra-assay reproducibility		Inter-assay reproducibility
		Ct ± SD	CV/%	Ct ± SD	CV/%
FAdV-4	2.84 × 102	24.70 ± 0.12	0.48	24.44 ± 0.17	0.70
	2.84 × 104	17.85 ± 0.26	1.47	17.91 ± 0.20	1.11
	2.84 × 106	11.28 ± 0.25	2.20	11.71 ± 0.29	2.47
DAdV-3	2.85 × 102	22.90 ± 0.24	1.05	22.96 ± 0.30	1.30
	2.85 × 104	18.28 ± 0.03	0.18	18.16 ± 0.24	1.34
	2.85 × 106	11.79 ± 0.12	1.03	11.89 ± 0.22	1.87