Fig. 7.

Paracrine effects of IGF-1 on YAP1 expression in fibroblasts. A Enzyme-linked immunosorbent assay (ELISA) for IGF-1 protein secretion in MCF-7 P and YS cancer cells. B Immunoblotting showing YAP and its canonical target genes CYR61 and CTGF in NFs treated ( +) or not (-) with MCF-7 P and YS-CM immunodepleted or not of IGF-1. The histograms represent the mean ± SD of three separate experiments in which band intensities were evaluated in terms of optical density arbitrary units (OD) and expressed as percentage of NF untreated cells (-) which were assumed to be 100%.. Each circle represents one independent experiments. Data were analyzed using one-way ANOVA with Tukey’s multiple comparisons post hoc test. Data are presented as mean values ± SD. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001