Fig. 1. Genes coexpressed with Of-E75A were screened by expression pattern.

(A) Heatmap displaying expression levels of genes coexpressed with E75A as determined by WGCNA clustering. All genes in this cluster show elevated expression levels at 24 to 36 hours AEL relative to the two other time points. Three replicates (R) were sequenced per time point. Each row represents a single gene, each column a different RNA sample. (B) Expression patterns of Of-E75A–coexpressed genes. Expression was visualized by in situ hybridization with digoxygenin-labeled probes in 24- to 48-hour AEL embryos. A subset of screened genes is shown here and the rest in fig. S2. (i) OFAS008150 is expressed in stripes in the anterior SAZ and broadly in the posterior SAZ, suggestive of a role in segmentation. (ii) OFAS013944 expression was observed ubiquitously in germ-band–stage embryos, (iii) OFAS000035 (annotated as H6-like-homeobox) expression was observed in specific domains of the head lobes, (iv) OFAS012059 expression was observed in the head lobes and along the midline, (v) OFAS025181 (annotated as tailup) expression was observed in a single dot in each head lobe, (vi) OFAS025116 (annotated as single-minded) expression was observed along the midline, (vii) OFAS025203 (annotated as Brachyury) expression was observed in the posterior tip of the SAZ, and (viii) OFAS003956 expression was observed ubiquitously. Genes are named on the basis of published annotations (42).