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. 2024 Nov 15;10(46):eadp5689. doi: 10.1126/sciadv.adp5689

Fig. 5. m6A modification promotes RNA accumulation in SGs.

Fig. 5.

(A) Representative time-lapse images of the FRET ratio of SMIS-WT and SMIS-3A after DOX induction for 12 hours and NaAsO2 treatment at the indicated time points. Scale bars, 5 μm. Normalized FRET ratio on granules in SMIS-WT (B) or SMIS-3A (C) after DOX induction for 12 hr and NaAsO2 treatment for 1 hour. One dot represents the average FRET ratio of granules per cell after NaAsO2 treatment normalized to the cytosolic FRET ratio in the same cell before NaAsO2 treatment. Unpaired t test with Welch’s correction (SMIS-WT: n = 10, P < 0.0001; SMIS-3A: n = 10, P < 0.0001). (D) MCP-3 × GFP and G3BP1 images in m6A reporter cells after transient transfection of m6A reporter cells with MCP-3×GFP for 36 hours, DOX induction for 12 hours, and NaAsO2 treatment for 1 hour. The white arrow shows the quantification path. Scale bar, 1 μm. (E) G3BP1 and MCP-3×GFP intensity profiles are shown along the arrow in (D). (F) G3BP1 and m6A immunostaining images in HeLa cells after NaAsO2 treatment for 1 hour. Scale bars, 5 μm. (G) MCP-3×GFP and G3BP1 images in m6A reporter cells after transient transfection with MCP-3×GFP for 12 hours, siSC or siMETTL3 treatment for 24 hours, DOX induction for 12 hours, and NaAsO2 treatment for 1 hour. Scale bars, 2 μm. (H) EGFP mean intensity in SGs, as indicated by G3BP1-GFP puncta, after siSC or siMETTL3 treatment from (G). Unpaired t test with Welch’s correction (siSC: n = 53; siMETTL3: n = 37; P < 0.0001).