Fig. 4. Actomyosin contractility mediates cell response to viscous energy dissipation.

(A) Representation of mechanical communication between the ECM and the cytoskeleton through molecular clutches of integrins and adaptor proteins like talin. Blebbistatin and cytochalasin D treatments inhibit myosin activity and actin polymerization, respectively. (B to D) Quantification of nuclear versus cytoplasmic YAP distribution, cell spreading, and nuclear area of cells seeded on protein matrices in control conditions. (E to G) Quantification of YAP translocation to the nucleus, cell spreading, and nuclear area of cells treated with 1 μM cytochalasin D. (H to J) Quantification of YAP translocation to the nucleus, cell spreading, and nuclear area of cells treated with 10 μM blebbistatin. A minimum of 40 cells coming from three independent experiments were analyzed for quantifications in (B) to (J). Data are presented as mean ± SEM, ordinary one-way ANOVA. n.s., not significant; *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001.