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. 2024 Sep 19;26(11):1903–1917. doi: 10.1038/s41556-024-01512-w

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a, Single focal planes of representative fed L1 expressing HIS-72/H3.3–GFP upon control (ctrl) or let-363 RNAi treatment. Insets: zoom of single nucleus of the indicated tissue. Hyp, hypoderm; int, intestine; mus, muscle. Inset scale bar, 2.5 μm. b, Quantification of the percentage of fed animals under control or let-363 RNAi, within the indicated categories for 3D chromatin organization in intestine. Data are shown as mean ± s.e.m. of three independent biological replicas. c, Line plots of the averaged single nuclei profile of HIS-72/H3.3–GFP from 72 intestinal, hypodermal or muscle nuclei of fed animals upon control and let-363 RNAi, showing the radial fluorescence intensity as a function of the relative distance from the nucleolus centre. Data are from three independent biological replicas. For let-363 RNAi, larvae in proportions to their relative abundance within the chromatin organization categories as in b, were analysed for all tissues. The shaded area represents the 95% confidence interval of the mean profile. Profiles with a single peak were compared to estimate the statistical significance of differences, as described in Methods. P values are given in Supplementary Table 2. d, As in a but showing fasted WT and raga-1^GF animals expressing HIS-73/H3.3–GFP. Insets: zoom of single nucleus of the intestine. e, Quantification of the percentage of fasted WT and raga-1^GF animals in the indicated categories for 3D chromatin organization in intestine. Data are shown as mean ± s.e.m. of three independent biological replicas. f, Line plots as in c but of the averaged single nuclei profile of HIS-72/H3.3–GFP from 72 intestinal nuclei of fasted WT and raga-1^GF larvae. Data are from three and four independent biological replicas for raga-1^GF and WT, respectively. For raga-1^GF, larvae in proportions to their relative abundance within the chromatin organization categories as in e, were analysed. For c and f, heat maps of single nuclei profiles are provided in Extended Data Fig. 3h,p, respectively. Source numerical data are available in Source data.

Source data