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. 2024 Oct 1;26(11):1934–1942. doi: 10.1038/s41556-024-01519-3

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 7 — a, Cartoon representation of a Drosophila ovariole showing the oogenesis stages analyzed for P-body formation in response to nutrient stress. b, Quantified intensities of oskar smFISH signal under nutrient-rich (0 h) and nutrient-poor (6 h) conditions in nurse cells and oocytes, indicating that oskar mRNA expression is not altered. Data are presented as mean values ± SD. ns = non-significant. For the statistical analysis ordinary one-way ANOVA applying Šídák’s multiple comparison method was used. Each black dot indicates a single egg chamber analyzed. Fluorescence intensity values of nurse cells and oocyte compartment were obtained from 12 egg chambers maintained under the nutrient-rich condition and from 12 egg chambers subjected to 6 hours of nutrient deprivation condition. c, smFISH detection of bicoid mRNA (magenta; top panel) or gurken mRNA (magenta; bottom panel) in EGFP-Me31B (green) expressing oskar WT flies (in absence of endogenous oskar RNA). Boxed areas are enlarged below the images. Representative line profiles (yellow) are plotted on the right. d, Bruno localizes to P-bodies upon nutrient deprivation, as evident from the colocalization of Me31B immunofluorescence (magenta) and BrunoKI-EGFP (green) signal. e, Co-detection of oskar WT (magenta) and EGFP-Me31B (green) under nutrient-poor conditions. Boxed areas are enlarged on the right. The graph shows the percentage (%) of egg chambers forming P-bodies (PB-positive) in wild-type (w¹¹¹⁸) and transgenic oskar WT flies (in the absence of endogenous oskar mRNA). Bar plot displays mean of the quantified data with error bars showing the standard deviation (±SD). Three independent biological replicates were analyzed and egg chambers at stages 6–9 were scored. Each biological replicate is indicated with a black circle and represents percentage of PB-positive egg chambers used for statistical comparison. N denotes a total number of egg chambers scored from all three biological replicates for w¹¹¹⁸ (N = 247) and oskar WT (N = 216) flies. P-value = ns (non-significant, p = 0.2178) is determined by two-tailed unpaired two-tailed Student’s t-test.

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