Table 1.

Application of nanofiber membrane in organ-on-a-chip construction [21,23,[28], [29], [30], [31], [32], [33], [34], [35]].

Type of chip	Materials	Nanofiber/membrane dimensions	Cell Type	Point	Ref
Lung-on-a-chip	PCL; PCL/collagen	/	A549	PCL/collagen nanofiber is better than PCL nanofiber suitable for application in lung-on-a-chip.	[21]
	PLGA	Thickness: ∼ 3 μm.	A549 HFL1 HUVEC	As substrate and 3D scaffolds for co-culture; The evaluation of gefitinib and the mechanism of drug resistance.	[28]
	PLGA/PDMS	Thickness: PLGA ∼ 3 μm; PDMS ∼70 μm; micropores diameter: 200 μm	NCI-H1650 NCI-H460	Realize the deformation of the membrane; study the sensitivity of gefitinib.	[29]
	Gelatin	Porosity: >50 %; Size: 1∼10 μm; Thickness: 100∼500 μm.	A549	Simulate the periodic movement of lung respiration in vivo.	[30]
	PCL/gelatin	Thickness: ∼20 μm.	A549 HUVEC HSAEC	Study how the extracellular matrix structure affects epithelial cell injury during airway reopening.	[31]
	PU	Average diameter: 420 ± 99.43 nm; Average 2D pore size: 1.35 μm ± 436 nm.	HAECs HMVEC-Ls	After applying several different types of mechanical forces, the degree of cell damage was observed and the recovery of cells was monitored.	[32]
Heart-on-a-chip	PLLA; PU	Average diameter: PLLA 186 ± 65 nm; PU 318 ± 102 nm.	MSC HCM H9C2	Study the effect of MSCs on mitochondrial dysfunction in HCM and H9C2 cells.	[33]
Liver-on-a-chip	PCL	Diameter: 600–1000 nm; Membrane porosity: 76 %; Pore size: 3 μm; Thickness: 113.7 ± 2.7 μm.	HepG2	As a scaffold for cell culture; monitor the secretion of albumin and alpha-fetoprotein in real time.	[23]
Nerve-on-a-chip)	PLLA	Fiber densities:11 %, 46 %, 71 %	DRG	Study the impacts of nanofibers with different densities on the vertical velocity of axonal growth and the recovery of the axotomized areas.	[34]
multi-organ-on-a-chip	PLLA/collagen I	Average diameter: 325 ± 50 nm; Average pore size: <5 μm.	HFF LO2 PC-9 HUVEC	Simulate the tissue-tissue interface; establish the model of jaundice in vitro.	[35]