Fig. 2.

αKlotho enhanced renal mitophagy in CI-AKI mice. (A). Heatmap of RNA-sequencing results comparing αKlotho-treated CI-AKI mice with vehicle-treated CI-AKI mice. (B) KEGG analysis in αKlotho-treated CI-AKI mice versus vehicle-treated CI-AKI mice. (C) Representative TEM images of mitophagosomes (red arrow) in renal tubular epithelial cells. Scale bar, 1 μm. (D and E) Immunoblot analysis of LC3B in the mitochondrial fraction of kidneys. (F and G) Representative images and quantification of co-staining of LC3B and VDAC in kidney tubules. Scale bar, 50 μm. Data were expressed as mean ± SEM. n = 4–5. **p < 0.01; ***p < 0.001