Fig. 3.

BNIP3 played a critical role in αKlotho-promoted mitophagy in CI-AKI mice. (A) Relative mRNA expressions of Bnip3 in the kidneys. (B and C) Immunoblot analysis of BNIP3 in the mitochondrial fraction of kidneys. (D and F) Representative images and quantification of BNIP3 by immunohistochemical staining in kidney tubules. Scale bar, 50 μm. (E, G and H) Immunoblot analysis of BNIP3 and LC3BII in the mitochondrial fraction of kidneys. (I and J) Representative images and quantification of co-staining of LC3B and VDAC in kidney tubules. Scale bar, 50 μm. Data were expressed as mean ± SEM. n = 4–5. *p < 0.05; **p < 0.01; ***p < 0.001