Fig. 4.

The protective effects of αKlotho against contrast media were abrogated in BNIP3 deficiency mice. (A and B) Representative histology images and pathological score of tubular injury by HE staining. Bottom panels are enlarged images of the boxed areas in the top panels. Scale bar, 50 μm. (C) Serum creatinine levels of different groups. (D-G) Immunoblot analysis of Bcl-2, Bax and cleaved caspase-3 in kidneys. (H and I) Representative images and quantification of TUNEL-positive cell by TUNEL staining in kidney tubules. Scale bar, 50 μm. Data were expressed as mean ± SEM. n = 4–5. **p < 0.01; ***p < 0.001