Fig. 5.

BNIP3 participated in αKlotho-mediated mitophagy in HK-2 cells under contrast media injury. (A-C) Immunoblot analysis of BNIP3 and LC3B in the mitochondrial fraction of HK-2 cells. (D) Representative images of mitophagy (yellow) by mitophagy dye (red) and lysosome dye (green) in HK-2 cells. Scale bar, 50 μm. (E-H) Immunoblot analysis of Bcl-2, Bax and cleaved caspase-3 in HK-2 cells. (I and J) Representative images and quantification of TUNEL staining in HK-2 cells. Scale bar, 50 μm. Data were expressed as mean ± SEM. N = 3. *p < 0.05; **p < 0.01; ***p < 0.001