Fig. 6.

αKlotho relieved mitochondrial injury and reduced ROS generation via BNIP3-mediated mitophagy. (A) Representative TEM images of mitochondrial morphology in renal tubular cells of WT and Bnip3^−/− mice. Scale bar, 1 μm. (B and C) Representative images and quantification of 8-OHDG by immunohistochemical staining in kidney tubules. Scale bar, 50 μm. (D and E) Immunoblot analysis of MnSOD in kidneys. (F and G) Representative images and quantification of MitoSOX staining in HK-2 cells. Scale bar, 50 μm. Data were expressed as mean ± SEM. N = 4–5. **p < 0.01; ***p < 0.001