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. 2024 Nov 15;14:28193. doi: 10.1038/s41598-024-79458-0

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

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Fig. 1 — The effect of hypoxia on lipid quantity and rTCA contribution to de novo lipogenesis during adipocyte differentiation. The effect of hypoxia on intracellular palmitate content in early and late adipocytes (A). Percentage of ¹³C derived via rTCA from ¹³C₅-labeled glutamine into intracellular palmitate (B). Percentage of ¹³C derived from ¹³C₁-labeled glutamine into citrate (C) and malate (D), representing the rTCA-generated metabolites. Data are presented as the mean ± SD, N = 9. Two-way ANOVA was performed followed by Tukey’s post-hoc test. ^*p < 0.05 for comparison 4% O₂ vs. 21% O₂; ^#p < 0.05 for comparison 7 Days vs. 14 Days.