Fig. 3.
Gene expression change of selected enzymes during adipocytes differentiation under hypoxia. Quantitative real-time PCR was employed to assess gene expression of (A_ pyruvate dehydrogenase E1 alpha 1 (PDHA1), (B) ATP citrate lyase (ACLY), and (C) acyl-CoA synthetase short-chain family member 2 (ACSS2), (D) isocitrate dehydrogenase 1 (IDH1), (E) isocitrate dehydrogenase 2 (IDH2). All values are expressed as 2^ΔCT. Data are presented as the mean ± SD, N = 6. Two-way ANOVA test was performed followed by Tukey’s post-hoc test.*p < 0.05 for comparison 4% O2 vs. 21% O2; #p < 0.05 for comparison 7 Days vs. 14 Days; ns = non-significant.