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. 2024 Nov 15;14:28174. doi: 10.1038/s41598-024-78077-z

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — Differentiation of hDPSCs and MT-hDPSCs into neurons assessed by β-Tubulin III (TUJ-1) staining. (A) At the site of injury, the nuclei of hDPSCs and MT-hDPSCs were labeled with DAPI, the cells themselves were labeled with DiI, and their neuron differentiation was labeled with β-tubulin III. (B) A comparison of the number of β-tubulin III-positive cells among the Vehicle (SCI + DMEM), SCI + hDPSCs, and SCI + MT-hDPSCs groups demonstrated that MT-hDPSCs differentiated into neurons significantly more than hDPSCs at the SCI site (**p < 0.01). hDPSCs human dental pulp stem cells, MT melatonin, SCI spinal cord injury; magnification: ×20.