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. 2024 Nov 16;14:28254. doi: 10.1038/s41598-024-79352-9

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Expression of Piezo in mammalian tissue. (A, B) Piezo1 is expressed in mammalian podocytes, which are visualized using the podocyte marker Synaptopodin. Piezo1 is expressed in areas close to the slit diaphragm and in the cell body (arrow heads). Scale bar = 10 μm and 5 μm for the magnification in row 2 and 3 (A) and 10 μm (B). Negative control: without primary antibodies. Scale bar = 10 μm. Antibody used for Piezo1: (A): Proteintech and (B): Novus, see Material and Methods for details. (C) Single nucleus RNAsequencing data from human diabetic kidney disease samples (DKD) and controls reveal Piezo1 expression in podocytes, which decreases in DKD. Data used from the Humphries lab with permission³⁴. PT: proximal tubule, PTVCAM1: VCAM1( +) proximal tubule, PEC: parietal epithelial cells, ATL: ascending thin limb, TAL1: CLDN16(-) thick ascending limb, Tal2: CLDN16( +) thick ascending limb, DCT1: early distal convoluted tubule, DT2: late distal convoluted tubule, PC: principal cells, ICA: type A intercalated cells, ICB: type B intercalated cells, PODO: podocytes, ENDO: endothelial cells, MES: mesangial cells and vascular smooth muscle cells, FIB: fibroblasts, LEUK: leukocytes.