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. 2024 Nov 16;14:28254. doi: 10.1038/s41598-024-79352-9

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Loss of Piezo causes a severe nephrocyte phenotype in adult pericardial cells. (A) Visualization of the nephrocyte diaphragm with Duf and Pyd antibodies reveals a significant decrease in nephrocyte diaphragm length in adult pericardial cells upon loss of Piezo at 25 °C (Piezo kd). Student’s t-test: **: p < 0.01. Scale bar = 5 μm. Control: w;sns-Gal4/ + ; + /UAS-dicer2; Piezo kd: w;sns-Gal4/ + ;UAS-piezo-RNAi/UAS-dicer2. (B) Immunofluorescence staining with a Duf and Pyd antibody reveals significant morphological changes in Piezo kd adult pericardial cells in comparison to control cells at 28 °C. Scale bar = 5 μm. Student’s t-test: ****: p < 0.0001. (C) FITC-Albumin uptake assays in adult pericardial cells show a significant reduction of FITC-Albumin uptake in Piezo kd cells at 25 °C. Scale bar = 25 μm. Student’s t-test: ****: p < 0.0001. (D) At 28 °C FITC-Albumin uptake assays show a significant filtration defect in adult pericardial Piezo kd cells. Scale bar = 25 μm. Student’s t-test: ***: p < 0.001.