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. 2024 Nov 16;14:28254. doi: 10.1038/s41598-024-79352-9

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — GsMTx4 treatment reverses the phenotypes observed in nephrocytes overexpressing Piezo wildtype. (A, B) Immunofluorescence staining with Duf and Pyd antibodies reveals a rescue of the morphological changes in adult pericardial cells expressing Piezo wildtype after incubation with 5 μM GsMTx4 for 5 min. Scale bar = 5 μm. 1way-ANOVA with Tukey’s multiple comparison test: *: p < 0.05; ***: p < 0.001. Control: w; sns-Gal4/ + ;UAS-dicer2/ + . Piezo WT: w; sns-Gal4/ + ;UAS-dicer2/UAS-piezo-flag. (C, D) FITC-Albumin uptake assays show a rescue of the observed phenotype in adult pericardial nephrocytes overexpressing Piezo wildtype after 5 min GsMTx4 treatment. Scale bar = 25 μm. 1way-ANOVA with Tukey’s multiple comparison test: *: p < 0.05. (E) Schematic summarising the data. First panel: Loss of Piezo causes a morphological and functional phenotype, which is most severe in adult pericardial cells at 28 °C. Second panel: Pharmacological activation of endogenous Piezo by Yoda1 treatment and applying mechanical force causes functional defects and increased Ca⁺⁺ influx. Third panel: Overexpression of wildtype Piezo in adult pericardial nephrocytes results in morphological and functional disturbances. These phenotypes seem to be mediated by the mechanosensitive channel activity of Piezo. Treatment of Piezo wildtype overexpressing nephrocytes with GsMTx4 reversed the observed phenotypes. All images have been created with BioRender.com.