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. 2024 Nov 16;15:9936. doi: 10.1038/s41467-024-54219-9

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

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Fig. 6 — a Effect of cefoxitin on growth of a P. aeruginosa PAO1 ampG knockout complemented with E. coli AmpG on low-copy plasmid pHERD30T. Leaky (uninduced) expression of WT ampG (green up-arrow) restored growth to WT levels, while K62A (orange diamond) or Y152A (blue square) mutants had the same expression as empty control (gray down-arrow). Induction of expression with 0.1% arabinose partially restored growth of Y152A but not K62A. b P. aeruginosa AmpG mutants tested as in a. K66A and Y159A affected growth similarly to the ampG knockout (coloring as in a). On induction with 0.1% arabinose, growth of K66A and Y159A was partially restored to varying degrees. c Growth of P. aeruginosa AmpG mutants as in b at varying levels of arabinose induction (gray down-arrow uninduced, blue circle 0.1%, green up-arrow 0.2%, orange diamond 0.5%). Both K66A and Y159A show a concentration-dependent increase in growth on induction, with the response much higher for Y159A. d Identification of a D74N mutant that rescues growth of the P. aeruginosa AmpG K66A mutant. (coloring as in a, blue square represents K66A/D74N). Experiments were performed in technical triplicate and biological duplicate with a representative shown. Data points correspond to technical replicates, with error bands representing SD.