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. 2024 Nov 16;15:9937. doi: 10.1038/s41467-024-54213-1

Fig. 5. Serum transacylases produce hemi-BMP and BMP in vitro and in vivo.

Fig. 5

A–C BMP, PG, and hemi-BMP synthesis by heparin-plasma of wild-type and Lipg-ko mice using sn-1-oleoyl LPG or di-oleoyl PG as substrate (n = 4). The reaction mix (50 µl total) contained 30 µl plasma, 0.5 mM substrate prepared in PBS (pH 7.4), and 1% FA-free BSA. Samples were incubated for 1 h at 37 °C. D–F Plasma PG, BMP, and hemi-BMP content of mice after intraperitoneal injection of di-oleoyl PG (wild-type n = 3; Lipg-ko n = 4) (5 mg/mouse). Blood samples of wild-type and Lipg-ko mice were collected before injection and at the indicated time points after injection (n = 3). G Total tissue BMP content of wild-type (n = 4) and Lipg-ko mice (n = 5). Data are presented as mean ± SD. Statistically significant differences were determined by unpaired two-tailed Student’s t-test, followed by corrections for multiple comparisons using the Holm-Sidak post hoc test. Source data are provided as a Source Data file.