Fig. 2.

A Scheme depicting the in vitro experimental design for investigating the effects of serum derived exosomes. B–E mRNA fold change levels of various proinflammatory genes in the cell lines BV-2 (microglia), C8-D1A (astrocytes) and bEnd.3 (brain endothelial cells) treated for 24 h with 0.1 mg/ml of exosomes derived from the serum of the donor mice that were injected with PBS (PBS Exo), LPS (LPS Exo) or LPS derived exosome supernatant (LPS SN) for 24 h, B Il1a C Tnf, D Il6, E Il1b (n = 3–4 male mice in each group, data represent mean ± SEM; two-way anova with Dunnett post-hoc test, *p < 0.05, **p < 0.01, ***p < 0.001)