Table 2.
Application of single-cell RNA sequencing in diabetic kidney disease
| Article information | Single-cell technique | Disease/model | Tissue type | Sample number | Cell number | |
|---|---|---|---|---|---|---|
| mouse | Fu et al. (2019) [46] | Fluidigm C1 Single-cell Auto Prep System & Illumina NextSeq 500 platform | STZ-induced diabetic eNOS−/− mice | Glomerular cells and immune cells |
Control (3) DKD (3) |
Total: 829 Control:403 DKD:426 |
| Chung et al. (2020) [48] | Chromium Single Cell 3’ Library and Gel Bead Kit; Illumina HiSeq 4,000 |
Nephrotoxic serum nephritis; DKD; Doxorubicin nephropathy; Podocyte-specific genetic disease |
Glomerular cells |
Control (3) Nephritis (4) DKD (6) Doxorubicin (2) CD2AP (2) |
Total: 74,149 Control: 5,488 Nephritis:1,936 |
|
| Fu et al. (2022) [66] | 10x Genomics Chromium and Illumina Novaseq | OVE26 mice | Immune cells |
Control (3) DKD mice (3) |
Total:17,000 Control: 10,500 DKD: 6,500 |
|
| Wu et al. (2022) [56] | 10x Genomics Chromium & Bulk RNA-seq | db/db with uninephrectomy | Kidney | Mice (70) | Total: 946,660 | |
| Wu et al. (2022) [56] | 10×Genomics Chromium and Illumina NovaSeq | db/db | Kidney |
db/db (8) db/db + dapa (8) db/db + irbe (8) db/db + dapa + irbe (8) |
Total: 83,585 | |
| Liu et al. (2023) [67] | ScRNA-seq & Bulk RNA-seq | BTBR ob/ob | Glomerular cells |
Control (8) Ob/ob (8) |
Total: 70,944 | |
| Animal and Human | Li et al. (2022) [68] | ScRNA-seq & snRNA-seq & Bulk RNA-seq | Wild-type mice, db/db mice | Glomerular, tubules cell | Wild-type mice db/db mice | / |
| DKD |
Control (3) DKD (3) |
|||||
| Tsai et al. (2023) [69] | ScRNA-seq | db/m mice, db/db mice | Blood, urine and kidney | db/m mice + db/db mice | Total: 6,775 | |
| Early DKD |
Control (24) T2D (48) |
|||||
| Lu et al. (2022) [57] | Illumina NovaSeq 6000 & Illumina HiSeq 4000 | DKD rat | Kidney |
Control (3) DKD rat (3) |
/ | |
| Early DKD | Control (3) DKD (3) | |||||
| Balzer et al. (2023) [70] | 10X Genomics, Qiagen, Agilent Technologies, Illumina, Meso Scale Discovery and Olink | ZSF1 rats | Kidney |
ZSF1 lean rat (6) ZSF1 obese rat (10) |
Total: 4,821 | |
| DKD |
Control & DKD (991) |
|||||
| Human | Menon et al. (2020) [71] | 10×Genomics Chromium and Illumina | Early DKD | Kidney biopsy |
Control (18) DKD (44) |
Total: 111,035 |
| COVID-19 | Urine | DKD (44) SARS-CoV-2 (13) | Total: 25,791 | |||
| Barwinska et al. (2021) [72] | LMD and sequenced and sn Drop RNA-seq | DKD | Kidney |
Control (9) DKD biopsy (6) |
Total: 24,387 | |
| Stefansson et al. (2022) [73] | ScRNA-seq | Early DKD, Hyperfiltration | Kidney |
Type 2 diabetes with HF (26) or pre-HF (26) |
\ | |
| Wilson et al. (2022) [74] | SnRNA-seq & snATAC-seq | DKD | Kidney cortex | Control (6) DKD (7c) | Total: 107,634 | |
| Hirohama et al. (2023) [75] | ScRNA-seq & Bulk RNA-seq | DKD | Kidney | Control (10) DKD (23) | Total: 64,333 | |
| Schaub et al. (2023) [76] | ScRNA-seq | Type 2 diabetes | Kidney | Control (6) Type 2 diabetes (16) | Total: 40,535 |
Abbreviations: scRNA-seq single-cell RNA sequencing, snRNA-seq single-nucleus RNA sequencing, Bulk RNA-seq bulk RNA sequencing, snATAC-seq single nucleus assay for transposase-accessible chromatin using sequencing, STZ streptozotocin, eNOS endothelial nitric oxide synthase, DKD diabetic kidney disease, BTBR black and tan, brachyuric