Table 1.

The Activities of the Novel Compounds 5a-r Over Normal Cells (WI-38) and 4 Cancer Cell Lines (Using SOR as a Reference) and the Values of Their IC₅₀ (μM) are Provided

Comp. no.*	R1	R2	n	IC50 (µM)**
				HCT-116	HePG-2	Hela	MCF-7	WI-38
5a	H	OCH3	0	62.70±3.6	81.29±4.3	83.41±4.4	79.47±3.9	29.42±2.1
5b	H	Cl	0	77.26±4.2	88.46±4.8	>100	92.19±4.7	64.31±3.7
5c	H	Br	0	48.22±3.0	57.82±3.6	67.67±3.9	55.15±3.2	>100
5d	H	CF3	0	6.09±2.2	2.39±0.1	8.94±0.7	4.81±0.2	36.29±2.4
5e	H	H	1	54.91±3.3	65.68±3.8	75.34±4.2	72.77±3.6	>100
5f	H	OCH3	1	14.78±1.3	34.24±2.4	31.51±2.3	18.66±1.5	91.40±5.1
5g	H	Cl	1	35.29±2.6	47.19±3.1	53.68±3.1	39.58±2.6	85.98±4.8
5h	H	H	2	5.89±0.3	6.74±0.5	11.61±0.9	13.88±1.1	41.06±2.6
5i	H	Cl	2	39.06±2.7	49.83±3.2	58.33±3.3	43.15±2.8	>100
5j	Cl	OCH3	0	9.51±0.9	12.34±1.0	19.10±1.4	16.01±1.3	38.52±2.5
5k	Cl	Cl	0	23.43±2.1	40.85±2.6	38.02±2.5	32.91±2.3	>100
5l	Cl	Br	0	28.64±2.3	44.74±2.9	49.41±2.9	37.25±2.5	>100
5m	Cl	CF3	0	18.14±1.5	30.68±2.2	27.88±2.1	21.45±1.8	76.27±4.3
5n	Cl	H	1	44.21±2.8	52.39±3.4	61.48±3.7	48.68±3.0	73.64±3.9
5o	Cl	OCH3	1	69.30±3.9	84.33±4.6	90.23±4.8	86.52±4.2	35.87±2.3
5p	Cl	Cl	1	8.32±0.7	9.72±0.7	14.27±1.2	7.99±0.5	56.87±3.3
5q	Cl	H	2	32.53±2.5	41.58±2.8	45.22±2.7	24.58±2.0	53.01±3.1
5r	Cl	Cl	2	13.20±1.1	17.67±1.4	22.36±1.7	10.74±0.9	74.17±4.1
SOR***	-	-	-	5.47±0.3	9.18±0.6	7.26±0.3	4.17±0.2	10.65±0.8

Note: *Symbols represent synthesized compounds. **The IC₅₀ value is the dose at which 50% of tumor cell growth is inhibited. The data is shown as the mean ± SD from the dose-response graphs in triplicate. IC₅₀ (mg/mL): 1–10 (very strong), 11–20 (strong), 21–50 (moderate), 51–100 (weak), 100–200 (very weak), above 200 (non-cytotoxic). ***SOR represents Sorafenib.