Figure 6. Vinculin controls collective migration upon wound healing.

Collective migration of MCF10A, KO, and KI-P878A cells over the wound was imaged by phase contrast over time and analyzed by particle image velocimetry. (A) Quantification of monolayer edge progression over time. (B) Quantification of monolayer edge speed over time. (C, D) Heat map representation of velocity ((C), length of displacement vectors) and local order parameter ((D), cosine of angles between adjacent displacement vectors). The vertical axis corresponds to coordinates along the perpendicular axis relative to the monolayer edge (edge position kept constant on the top of heat maps), whereas the horizontal axis corresponds to time course (from left to right). N = 3 with similar results; 1 representative experiment shown.