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Activity of the UBP1 analogues, UBPDQL and UBPD2QL, as revealed by SDS electrophoresis in 12% polyacrylamide gel. 1 and 2 – the substrate 6xHisUBI::S digested with UBPDQL and UBPD2QL, respectively; 3 – the undigested substrate, 6xHisUBI::S; 4 – molecular mass marker (kDa); 5 and 6 – the substrate UBI::K digested with UBPD2QL and UBPDQL; 7 – the undigested substrate, UBI::K.
