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Binary complex formation of CARD protein complex in rabbit reticulocyte lysates. Equal amounts of in vitro expressed proteins were incubated in rabbit reticulocyte lysates at 4°C for 1 hour. After incubation, protein and complexes were immunoprecipitated with anti-6XHis antibody. The precipitated samples (lane 2) together with the individually-expressed proteins (lane 1 and 3) were detected by western blot using anti-GST antibody.
